Berkeley Lab researchers are developing molecular imaging probes and techniques to study metals in the brain that have been linked to disorders such as Alzheimer’s and Parkinson’s diseases.
Berkeley Lab Researchers Demonstrate First Size-based Chromatography Technique for the Study of Living Cells
Using nanodot technology, Berkeley Lab researchers demonstrated the first size-based form of chromatography for studying the membranes of living cells. This unique physical approach to probing cellular membrane structures reveals critical information that can’t be obtained through conventional microscopy.
Berkeley researchers have answered a central question about Cas9, an enzyme that plays an essential role in the bacterial immune system and is fast becoming a valuable tool for genetic engineering: How is Cas9 able to precisely discriminate between non-self DNA that must be degraded and self DNA that may be almost identical within genomes that are millions to billions of base pairs long.
Through a combination of water, oil and nanoparticle surfactants plus an external field, spherical droplets are being transformed into ellipsoids and other unusual shapes that could find many valuable uses.
Combining components of Rosetta and PHENIX, two successful software programs for creating 3D structural models of proteins and other biomolecules, Berkeley Lab researchers have created a new method for refining those models and making the best of available experimental data.
By preventing the build-up of toxic metabolites in engineered microbes, a dynamic regulatory system developed at JBEI can help boost production of an advanced biofuel, a therapeutic drug, or other valuable chemical products. The system has already been used to double the production in E. coli of amorphadiene, a precursor to the premier antimalarial drug artemisinin.
Through a combination of transmission electron microscopy (TEM) and a unique graphene liquid cell, Berkeley Lab researchers have recorded the three-dimensional motion of DNA connected to gold nanocrystals, the first reported use of TEM for 3D dynamic imaging of soft materials.
Berkeley Lab researchers have shown that, contrary to the scientific axiom that only opposite charges attract, when hydrated in water, positively charged ions can pair up with one another.
A multi-institutional collaboration led by researchers with the Joint BioEnergy Institute (JBEI) and Joint Genome Institute (JGI) has developed a promising technique for identifying microbial enzymes that can effectively deconstruct biomass into fuel sugars under refinery processing conditions.