In the search for enzymes that can break lignocellulose down into biofuel sugars under the extreme conditions of a refinery, chemist Douglas Clark prospects for extremophilic microbes and engineers cellulases of his own.
How Sweet It Is: New Tool for Characterizing Plant Sugar Transporters Developed at Joint BioEnergy Institute
JBEI researchers have developed a powerful new tool that can help advance the genetic engineering of “fuel” crops for clean, green and renewable bioenergy – an assay that enables scientists to identify and characterize the function of nucleotide sugar transporters, critical components in the biosynthesis of plant cell walls.
Berkeley Lab has won three 2014 R&D 100 awards. This year’s winners include a fast way to analyze the chemical composition of cells, a suite of genetic tools to improve crops, and a method to screen images of 3-D cell cultures for cancer cells. The technologies could lead to advances in biofuels, food crops, drug development, and biomaterials, and a to better understanding of microbial communities, to name a few potential benefits.
The JBEI GT Collection, the first glycosyltransferase clone collection specifically targeted for the study of plant cell wall biosynthesis, is expected to drive basic scientific understanding of GTs and better enable the manipulation of plant cell walls for the production of biofuels and other chemical products.
Resistance is Not Futile: Joint BioEnergy Institute Researchers Engineer Resistance to Ionic Liquids in Biofuel Microbes
Researchers with the Joint BioEnergy Institute (JBEI) have identified the genetic origins of a microbial resistance to ionic liquids and successfully introduced this resistance into a strain of E. coli bacteria for the production of advanced biofuels.
Researchers at the Joint BioEnergy Institute (JBEI) have identified a rain forest microbe that feasts on the lignin in plant leaf litter, making it a potential ally for the cost-effective production of advanced biofuels.
By preventing the build-up of toxic metabolites in engineered microbes, a dynamic regulatory system developed at JBEI can help boost production of an advanced biofuel, a therapeutic drug, or other valuable chemical products. The system has already been used to double the production in E. coli of amorphadiene, a precursor to the premier antimalarial drug artemisinin.