A powerful scientific tool for editing the DNA instructions in a genome can now also be applied to RNA as Berkeley Lab researchers have demonstrated a means by which the CRISPR/Cas9 protein complex can be programmed to recognize and cleave RNA at sequence-specific target sites.
Life-Saving Dividends for Synthetic Biology Research: Microbial-Based Antimalarial Drug Shipped to Africa
The JBEI GT Collection, the first glycosyltransferase clone collection specifically targeted for the study of plant cell wall biosynthesis, is expected to drive basic scientific understanding of GTs and better enable the manipulation of plant cell walls for the production of biofuels and other chemical products.
Adam Arkin, director of Berkeley Lab’s Physical Biosciences Division, has been named one of six recipients of the 2013 Ernest Orlando Lawrence Award by U.S. Energy Secretary Ernest Moniz.
The Berkeley Open Biofoundry – BOB – is a Berkeley Lab proposal to DARPA aimed at providing the science and technology that will enable the engineering of biological systems to produce valuable chemical products on a commercial scale.
Resistance is Not Futile: Joint BioEnergy Institute Researchers Engineer Resistance to Ionic Liquids in Biofuel Microbes
Researchers with the Joint BioEnergy Institute (JBEI) have identified the genetic origins of a microbial resistance to ionic liquids and successfully introduced this resistance into a strain of E. coli bacteria for the production of advanced biofuels.
Berkeley Lab researchers led the development of a new technique for identifying gene enhancers – sequences of DNA that act to amplify the expression of a specific gene – in the genomes of humans and other mammals. Called SIF-seq, this new technique complements existing genomic tools, such as ChIP-seq, and offers additional benefits.
A collaboration led by Berkeley Lab’s Jennifer Doudna and Eva Nogales has produced the first detailed look at the 3D structure of the Cas9 enzyme and how it partners with guide RNA to interact with target DNA. The results should enhance Cas9’s value and versatility as a genome-editing tool.
Berkeley researchers have answered a central question about Cas9, an enzyme that plays an essential role in the bacterial immune system and is fast becoming a valuable tool for genetic engineering: How is Cas9 able to precisely discriminate between non-self DNA that must be degraded and self DNA that may be almost identical within genomes that are millions to billions of base pairs long.